24 HOUR URINE COLLECTION REQUIREMENTS

Test

Preservative

Aliquot

     

Aldosterone Urine

25ml 50% acetic acid

50ml (pediatric:20ml)

 

<5 yrs:15ml 50% acetic acid

Adjust ph to 2.0-4.0 with 50% acetic acid.

 

 

 

Amylase, Urine

No Preservative

20ml Refrigerate

 

Refrigerate during collection. Indicate if 12 hr collection.

 

 

 

 

Calcium, Urine

No Preservative.

20ml Refrigerate

 

Refrigerate during collection.

 

 

 

 

Catecholamine, Fractionation, Urine

25ml 50% acetic acid      <5yrs:15ml 50% acetic acid

50ml Adjust ph to 2.0-4.0 with 50% acetic acid.

 

Can collect with VMA and Metanephrines. Discontinue Mardelamine 48 hrs prior to collection.

 

 

 

 

Cortisol Free, Urine

25ml 50% acetic acid

50ml

 

<5 yrs:15ml 50% acetic acid

Adjust ph to 2.0-4.0 with acetic acid

 

 

 

Creatinine Clearance, Urine

No preservative.

10ml Refrigerate

 

Refrigerate during collection. Need serum creatinine also.

 

 

 

 

Creatinine, Urine

No preservative.

10ml Refrigerate

 

Refrigerate during collection.

 

 

 

Cystine, Urine

No preservative.

20ml Freeze

 

Refrigerate during collection. Collect before IVP.

 

 

 

 

Galactose, Urine

No preservative.

10ml Freeze

 

Random urine only.

Pediatric 5ml

 

 

 

Glucose, Urine

No preservative.

10ml Refrigerate

 

Refrigerate during collection.

 

 

 

 

HIAA-5 (Serotonin), Urine

25ml 50% acetic acid

20ml Adjust ph to 2.0-4.0 with 50% acetic acid. Refrigerate.

 

<5 yrs:15ml 50% acetic acid. See dietary restrictions in Lab Test Index.

 

 

 

 

Immunoelectrophoresis, Urine

No preservative.

100 ml Refrigerate

 

Refrigerate during collection.

 

 

 

 

17 Ketogenic Steroids / 17

25ml 50% acetic acid

50ml Adjust ph to 2.0-4.0 with 50% acetic acid.

Ketosteroids, Urine

<5 yrs:15ml 50% acetic acid

 

 

 

 

17 Ketosteroid Fractionation, Urine

25ml 50% acetic acid

50ml Adjust ph to 2.0-4.0 with 50% acetic acid.

 

<5 yrs:15ml 50% acetic acid

 

 

 

 

Magnesium, Urine

Collect & send in clean plastic urine containers with no metal caps or glued insterts.

10ml Send in metal free container

 

 

Refrigerate.

 

 

 

Mercury, Urine

Collect & send in clean plastic urine containers with no metal caps or glued insterts.

10ml Send in metal free container

 

 

Refrigerate.

 

 

 

Metals Heavy/Essential Elements,

Collect & send in clean plastic urine containers with no metal caps or glued insterts.

25ml Send in metal free container

Urine

 

Refrigerate.

 

 

 

Metanephrines, Urine

25ml 50% acetic acid         <5yrs:15ml 50% acetic acid

50ml

 

May collect with Catacholamines and VMA.

 

 

 

 

Oxalate, Urine

Patient should avoid taking large amounts of Vitamin C during collection.*Specimens sent with metal caps will
not be tested.

Add 30ml 6N HCL. At end of collection or 4 hour after.Adjust ph to 1.5-2.0 with 6N HCL. Send in metal free container. Refrigerate.

 

 

 

Phosphorus, Urine

No preservative.

10ml Refrigerate

 

Refrigerate during collection.

 

 

 

 

Porphyrin, Urine

5gm Sodium Carbonate.Protect from light. Do notsubstitute Sodium Bicarbonate. Refrigerate during collection.

50ml Refrigerate

 

 

 

 

 

 

Protein Electrophoresis, Urine

No preservative.

50ml Refrigerate

 

Refrigerate during collection.

 

 

 

 

Protein Total, Urine

No preservative.

10ml Refrigerate

 

Refrigerate during collection.

 

 

 

 

Sodium/Potassium, Urine

No preservative.

10ml Refrigerate

 

Refrigerate during collection.

 

 

 

 

Urea Nitrogen Clearance, Urine

No preservative.Need serum BUN collected also.

20ml Refrigerate

 

Refrigerate during collection.

 

 

 

 

Urea Nitrogen, Urine

No preservative.

10ml Refrigerate

 

Refrigerate during collection.

 

 

 

 

Uric Acid, Urine

No preservative.May be collected with Calcium and

10ml Refrigerate

 

Phosphorus.Refrigerate during collection.

 

 

 

 

VMA, Urine

25ml 50% acetic acid         <5yrs:15ml 50% acetic acid

50ml Refrigerate

 

Amoxicillin interferes: discontinue 3 days before collection

 

 

Can collect with Catacholamines and VMA.

 

Xylose, Urine

No preservative.Special collection instructions.

100ml Freeze

 

Patient must be NPO. 5 hr collection.