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Section-V
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POWERCHART NAME
|
VALPROIC ACID (DEPAKENE) LEVEL
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|
MERCY TEST NAME
|
VALPRO ACID
|
MERCY LAB CODE
|
VAL
|
| Specimen: |
- 0.5 ml lithium heparin plasma from a PST tube.
- Sodium Heparin, serum and EDTA plasma tubes are also accepted.
- Stable 48 hours refrigerated.
- Collection time is not critical.
|
| Comment: |
Peak reached 1-4 hours after oral dose. Indicate time last dose in comment. |
| Performed: |
Within 8 hours of receipt. Available stat. |
| Reference value: |
Therapeutic range: 50-100 mcg/ml Biopolar disorder therapeutic range: 50-125 mcg/ml
|
| Method: |
Emit Enzyme Immunoassay |
| CPT Code: |
80164 |
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POWERCHART NAME
|
VANCOMYCIN PEAK LEVEL
|
|
MERCY TEST NAME
|
VANCOMYCIN PEAK
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MERCY LAB CODE
|
VNPK
|
| Specimen: |
- 0.5 ml lithium heparin plasma from a PST tube.
- Serum, Sodium Heparin and EDTA plasma tubes are also acceptable.
- Stable 48 hours refrigerated
- If receipt of specimen is delayed by >48 hours specimen can be frozen
|
| Comment: |
Consult Pharmacy to establish collection time. Indicate time last dose in comment. |
| Performed: |
Within 8 hours of receipt. |
| Reference value: |
25-40 mcg/mL |
| Method: |
Emit Enzyme Immunoassay |
| CPT Code: |
80202 |
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POWERCHART NAME
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VANCOMYCIN LEVEL INTERMEDIATE
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MERCY TEST NAME
|
VANCOMYCIN INT
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MERCY LAB CODE
|
VNI
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| Specimen: |
- 0.5 ml lithium heparin plasma from a PST tube.
- Serum, Sodium Heparin and EDTA plasma tubes are also acceptable.
- Stable 48 hours refrigerated
- If receipt of specimen is delayed by >48 hours specimen can be frozen
|
| Comment: |
Consult Pharmacy to establish collection time. Indicate time last dose in comment. |
| Performed: |
Within 8 hours of receipt. |
| Reference value: |
Therapeutic range: 18-25 mcg/ml |
| Method: |
Emit Enzyme Immunoassay |
| CPT Code: |
80202 |
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POWERCHART NAME
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VANCOMYCIN TROUGH LEVEL
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|
MERCY TEST NAME
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VANCOMYCIN TRGH
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MERCY LAB CODE
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VNTR
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| Specimen: |
- 0.5 ml lithium heparin plasma from a PST tube.
- Serum, Sodium Heparin and EDTA plasma tubes are also acceptable.
- Stable 48 hours refrigerated
- If receipt of specimen is delayed by >48 hours specimen can be frozen
|
| Comment: |
Consult Pharmacy to establish collection time. Indicate time last dose in comment. |
| Performed: |
Within 8 hours of receipt. |
| Reference value: |
Recommendation of Pharmacy and Therapeutic Committee
- 10-15 mcg/ml Trough target range for mild infections (UTI, skin and soft tissue).
- 15-20 mcg/ml Trough target range for severe and life threatening infections (bacteremia, pneumonia, meningitis, endocarditis, osteomyelitis, diskitis, abscess, and joint infection.
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| Method: |
Emit Enzyme Immunoassay |
| CPT Code: |
80202 |
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POWERCHART NAME
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VAP (VERTICAL AUTO PROFILE) LIPID PROFILE
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MERCY TEST NAME
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VAP LIPID PROFILE*
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MERCY LAB CODE
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VAP
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| Specimen: |
3 ml serum from a SST tube. Refrigerate. |
| Coment: |
Specimen will be rejected if hemolyzed. |
| Performed: |
Test results will be sent to physician within 72 hours of specimen receipt at Atherotech Laboratories. |
| Reference value: |
Included on report. |
| CPT Code: |
|
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TEST NAME
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DERMAL, Herpes Simplex Virus & Varicella Zoster Virus, DNA Detection by PCR*
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See: Herpes Simplex Virus & Varicella Zoster Virus, DNA Detection by PCR - DERMAL
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POWERCHART NAME
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VARICELLA ZOSTER ANTIBODY IgG
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MERCY TEST NAME
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VARIC ZOST IgG*
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MERCY LAB CODE
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VZVG
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| Specimen: |
1.0 ml serum from a SST or plain red top tube. (0.3 ml minimum) |
| Comment: |
Qualitative testing only. Determines immunity status. For diagnosis of recent infection, testing of IgM and IgG antibodies are recommended. (VZGM) |
| Processing: |
Refrigerate up to 48 hours. > 48 hours freeze specimen. |
| Performed: |
Monday and Thursday 0800 cutoff |
| Reference value: |
Non-immune - presumed non-immune to VZV infection |
| Method: |
EIA |
| CPT Code: |
86787 |
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POWERCHART NAME
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VARICELLA ZOSTER ANTIBODY IgG IgM
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MERCY TEST NAME
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VARIC ZOST IgG IgM*
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MERCY LAB CODE
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CMIS
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| Specimen: |
1 ml serum from a SST or plain red top tube. |
| Comment: |
Includes IgG and IgM reslults |
| Processing: |
Send in screw-capped, round bottem, plastic vial, refrigerated to Mayo. Mayo VZGM |
| Performed: |
Test set up Monday through Saturday |
| Reference value: |
Included with Results |
| Method: |
IgG: Multiplex Flow Immunoassay (MFI) IgM: Immunofluorescence Assay (IFA)
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| CPT Code: |
86787 x2 |
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POWERCHART NAME
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NEUTROPHIL CYTOPLASM ANTIBODY VASCULITIS PANEL |
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MERCY TEST NAME
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VASCULITIS ANCA PANEL*
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MERCY LAB CODE
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VAPNL
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| Specimen: |
1.0 mL serum from a plain red top tube. |
|
Processing:
|
Send refrigerated to Mayo. Mayo # 83012 |
| Performed: |
3 days analytic time. |
| Reference value: |
- Reference ranges included with results.
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| Method: |
Multiplex flow immunoassay.
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| CPT Code: |
83516 Myeloperoxidase antibodies 83516 Proteinase 3 Antibodies 86255 Cytoplasmic Neutrophil Antibodies (ANCA) Screen - if appropriate 86256 Cytoplasmic Neutrophil Antibodies (ANCA) Titer - if appropriate. |
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TEST NAME
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VDRL, serum
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See: SYPHL
|
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POWERCHART NAME
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VDRL CSF QUALITATIVE
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MERCY TEST NAME
|
VDRL CSF*
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MERCY LAB CODE
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VDRC
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| Specimen: |
0.5 ml spinal fluid in a CSF tube. |
| Processing: |
Send frozen to Mayo. Mayo # 9028. |
| Performed: |
1 day. Test set up Monday through Saturday. |
| Reference value: |
Negative. |
| Method: |
Flocculation/Agglutination |
| CPT Code: |
86592 |
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POWERCHART NAME
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CULTURE VIRUS NON-RESPIRATORY
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MERCY TEST NAME
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NON RESP VIRUS*
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MERCY LAB CODE
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VRSNR
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Order:
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Specify site when ordering.
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Specimen:
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Body Fluid or Cerebrospinal Fluid (CSF): Send 1.0 mL in a sterile screw-capped vial. Send to Mercy lab immediately. Ocular or Rectal swabs: Collect sample on a Dacron-tipped swab with a plastic handle. Place swab in M5 transport media. Send to Mercy lab immediately. Stool: 5 – 10 g of stool. Collect in a sterile screw-capped container. Send to Mercy lab immediately. Tissue (Brain, Colon, Kidney, Liver): Place tissue in 1-2 mL of sterile saline in a sterile screw-capped container or use a M5 transporter. Send to Mercy lab immediately. Urine (Acceptable for Mumps testing ONLY): Collect 0.5 mL of urine in a sterile screw-capped container. Send to Mercy lab immediately.
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Comments:
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Blood, Bone marrow, Dermal, Genital, Oral or Urine specimens are not acceptable for viral culture. Dermal or oral specimens for hand-foot-and-mouth disease or enterovirus must clearly indicate this on the order.
- Urine and Bone Marrow specimens for CMV are not acceptable for viral culture. See Mayo test #81240 CMV by Rapid PCR
- Dermal or Genital specimens are not acceptable for viral culture. For requests for HSV/varicella zoster virus, see:
#82048 HSV and VZV PCR, Dermal #81241 Varicella-Zoster Virus, PCR #80575 HSV, PCR
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RL Client Comments:
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- Write VIRUS CULTURE, NON-RESPIRATORY (Mayo order code-VIRNR) on order form. Indicate specimen source.
- Send ALL specimens refrigerated to Mercy lab.
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Processing:
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Deliver to the lab immediately or refrigerate specimen. Send refrigerated to Mayo. Mayo order code-VIRNR.
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Performed:
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Test setup daily, Final report 2 weeks.
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Reference Value:
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Negative If positve, virus is identified.
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Method:
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Cell culture
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CPT Code:
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87252 Tissue Culture Inoculation 87254 Shell vial (if appropriate) 87176 Homogenization, tissue (if appropriate)
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POWERCHART NAME
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CULTURE VIRUS RESPIRATORY
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MERCY TEST NAME
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RESP VIRUS*
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MERCY LAB CODE
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VRSRSP
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Order:
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Specify collection site when ordering.
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Specimen:
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Throat Swabs: Collect specimen on a routine culturette. Send culturette to Mercy lab immediately.
Sputum, Bronchoalveolar lavage, Bronchial washings, Tracheal aspirate or Secretions, Nasal washings: Collect 1.0 mL of specimen. Place specimen in a sterile screw-capped container. Send to Mercy lab immediately.
Tissue (Lung and others): Place the tissue specimen in 1-2 mL sterile saline in a sterile screw-capped container or use a M5 transporter (green cap). Send to Mercy lab immediately.
Mumps testing , swab specimens for Mumps must clearly indicate "MUMPS" on request form to insure proper handling and test setup.
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Comments:
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Oral specimen is not acceptable for viral culture (unless parotid gland, secretion or client requests enterovirus). Clients with oral specimens for suspected hand-foot-and-mouth disease or enterovirus must order VIRNR / 87266 Viral Culture, Non-Respiratory and clearly indicate enterovirus on order.
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RL Client Comments:
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- Write VIRAL CULTURE, RESPIRATORY (Mayo order code-VRESP) on order form. Indicate specimen source.
- Send ALL specimens refrigerated to Mercy lab.
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Processing:
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Deliver to the lab immediately or refrigerate specimen. Send refrigerated to Mayo. Mayo order code-VRESP.
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Performed:
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Test setup daily, Final report 2 weeks.
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Reference Value:
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Negative If positive, virus is identified.
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Method:
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Cell culture
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CPT Code:
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87252 Tissue Culture Inoculation 87254 Shell vial (if appropriate) 87176 Homogenization, tissue ( if appropriate)
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POWERCHART NAME
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VISCOSITY
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MERCY TEST NAME
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VISCOSITY SERUM*
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MERCY LAB CODE
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VSCT
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| Specimen: |
1.5 ml serum from a red top tube. Keep specimen at 37°C until after centrifugation and separation of cells. |
| Processing: |
Send to Mayo. Mayo # 8168. |
| Performed: |
1 day. Test set up Monday through Friday. |
| Reference value: |
0 - 1.5 CPOISE |
| Method: |
Sonoclot Coagulation Analyzer. |
| CPT Code: |
85810 |
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POWERCHART NAME
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VITAMIN A (Retinol) LEVEL |
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MERCY TEST NAME
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VITAMIN A*
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MERCY LAB CODE
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VITA
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| Specimen: |
0.5 mL serum in amber vial. |
| Collection: |
Specimen must be collected in a plain red-top tube following an overnight (12 - 14 hour) fast. Patient may not consume any alcohol or injest any vitamin supplements for 24 hours prior to collection. infants - draw prior to next feeding.
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| Processing: |
Send serum refrigerated in an amber vial. Mayo #60298 / VITA
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| Cause for Rejection: |
Specimen collected in serum gel tubes are not accepted. |
| Performed: |
Monday through Friday; first shift |
| Method: |
Liquid Chromatography-Tandem mass Spectrometry (LC-MS/MS) |
| CPT Code: |
84590 |
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POWERCHART NAME
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VITAMIN B1 (THIAMIN) WHOLE BLOOD |
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MERCY TEST NAME
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THIAMIN VIT B1*
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MERCY LAB CODE
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VB1
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| Specimen: |
4 ml Whole Blood from a EDTA tube following an overnight (12 hour) fast. Immediately place specimen on wet ice and protect from light by wrapping specimen in aluminum foil. |
| Processing: |
Maintain specimen on wet ice and process within 4 hours of draw. Process by transfering whole blood into amber vial to protect from light. Specimen must be frozen within 4 hours of collect time. Mayo 85753/TDP
RL Clients: If you do not have amber vial, specimen may be frozen in EDTA tube and tube wrapped in aluminum foil.
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| Cause for Rejection: |
Specimens other than whole blood. Specimens not protected from light. |
| Performed: |
Monday through Friday. |
| Reference value: |
Reference ranges included with result
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| Method: |
High-Performance Liquid Chromatography (HPLC) with Flourescence |
| CPT Code: |
84425 Vitamin B1 |
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POWERCHART NAME
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VITAMIN B12 LEVEL
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MERCY TEST NAME
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VITAMIN B12
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MERCY LAB CODE
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B12
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| Specimen: |
0.5 ml serum from a SST tube. Aliquot specimen. |
| Comment: |
Collect before blood transfusion. If adequate amount of specimen and the result is below the reference range, a sample will be frozen for 14 days for possible additional testing. |
| Cause for rejection: |
Hemolyzed specimen not acceptable. |
| Processing: |
Avoid exposure to sunlight. Keep all SST tubes upright. Any inverted spun SST tubes must be aliquoted and re-centrifuged. Stable 8 hours at room temperature. Stable 24 hours refrigerated. Freeze if testing is not completed within 24 hours of collection.
Regional Lab Clients: Send in separate aliquot tube, not SST tube. Freeze if not received at Mercy Medical Center - North Iowa Laboratory within 24 hours of collection.
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| Performed: |
Within 8 hours of receipt. |
| Reference value: |
Male & Female: 180-914 pg/ml Indeterminate Range: 146 - 179 pg/ml Deficient Rance: 0 - 145 pg/ml
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| Method: |
Competitive Binding Immunoassay Chemiluminescent |
| CPT Code: |
82607 Vit B12 |
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POWERCHART NAME
|
VITAMIN B12 AND FOLATE LEVEL
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MERCY TEST NAME
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VIT B12 FOLATE
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MERCY LAB CODE
|
B12F
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| Specimen: |
0.5 ml serum from a SST tube. Aliquot specimen. Specimen must be collected prior to blood transfusion. |
| Comment: |
Collect before blood transfusion. Folate should not be ordered for patients who have recently received a radioisotope, methotrexate, or other folic acid antagonist. If adequate amount of specimen and the result is below the reference range, a sample will be frozen for 14 days for possible additional testing.
Folate reference range based on populations with folic acid fortification of foods. Deficient folate concentrations are considered to be less than 4ng/mL.
|
| Cause for rejection: |
Hemolyzed specimen not acceptable. |
| Processing: |
Avoid exposure to sunlight. Keep all SST tubes upright. Any inverted spun SST tubes must be aliquoted and re-centrifuged. Stable 8 hours refrigerated. Freeze if testing is not completed within 8 hours of collection.
Regional Lab Clients: Freeze if not received at Mercy Medical Center - North Iowa Laboratory within 8 hours of collection.
|
| Performed: |
Within 8 hours of receipt. |
| Reference value: |
Vitamin B12 Male & Female: 180 - 914 pg/mL Folate Male & Female: 5.9->24.8 ng/mL Indeterminate Range B12: 146 - 179 pg/mL
Deficient Range B12: 0 - 145 pg/mL
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| Method: |
Competitive Binding Immunoassay Chemiluminescent |
| CPT Code: |
82607 Vit B12+ 82746 Folate+
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POWERCHART NAME
|
VITAMIN D 25 HYDROXY LEVEL
|
|
MERCY TEST NAME
|
VIT D, 25-HYDROXY*
|
MERCY LAB CODE
|
VD25H
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| Comment: |
Total 25-Hydroxyvitamin D (Sum of D2 + D3) |
| Specimen: |
1 ml serum from SST gel or serum aliquoted from plain red tube. Minimum volume is 0.50 ml.
|
| Processing: |
Specimen is to be sent refrigerated. |
| Performed: |
Daily |
| Reference Value: |
<10 ng/mL (severe deficiency) 10-24 ng/mL (mild to moderate deficiency) 25-80 ng/mL (optimum levels) >80 ng/mL (toxicity possible)
|
| Method: |
CMIA. |
| CPT Code: |
82306
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POWERCHART NAME
|
VMA URINE
|
|
MERCY TEST NAME
|
VMA 24 UR*
|
MERCY LAB CODE
|
VVMA
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| Comment: |
This assay is of most value when the specimen is collected during a hypertensive episode. For children 14 years old and younger, Mayo #9254 (VMA and Homovanillic Acid, Pediatric uring) is the recommended test.
A single 24-hour urine collection may be used for CATECHOLAMINE FRACTIONATION, METANEPHRINES [METN24U] and VMA [VMA24UR]. The specimen must be kept refrigerated during collection.
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| Patient preparation: |
Amoxicillin interferes. Discontinue for 3 days prior to collection. The drug Mandelamine interferes with the test procedure and should be discontinued 48 hours prior to collection of the specimen.
|
| Specimen: |
Before start of collection, add 25 ml 50% acetic acid preservative to the container (15 ml 50% acetic acid for children <5 years old). RL clients, please call Mercy Lab to have a jug prepared with preservative. Refrigerate during collection.
|
| Reference Lab |
Adjust pH to 2.0-4.0 with 50% acetic acid. Aliquot 20 ml and indicate the 24-hour volume. |
| Processing: |
Separate aliquots must be submitted for Metanephrines and Catecholamines if collected with this specimen. Identify which specimen is for VMA. Mayo #9454
|
| Performed: |
2 days. Test set up Monday through Saturday. |
| Mercy lab Processing: |
Send 5 ml in a 13 ml urine tube to Mayo refrigerated. |
| Reference value: |
Included on report. |
| Method: |
Liquid Chromatography-tandam mass spectrometry (LC-MS/MS) |
| CPT Code: |
84585 VMA UR+
|
|
POWERCHART NAME
|
FACTOR VIII VON WILLEBRAND ANTIGEN
|
|
MERCY TEST NAME
|
VON WILL FACT AG*
|
MERCY LAB CODE
|
VONW
|
| Specimen: |
1 ml plasma from blue top tube filled appropriately with amount of blood listed on the label. |
| Processing: |
Centrifuge, remove plasma, spin plasma again, aliquot to a new plastic tube. Freeze plasma in plastic vial. Send frozen to Mayo. Order VonWillebrand Antigen Mayo # 9051 / VWAG.. Complete a “Mayo Additional Test Information Form” (Fill out the Coagulation portion of this form), or a “Coagulation Form” and send with the specimen.
Double spin coagulation specimens to ensure that all platelets are removed: 1. Centrifuge specimen. Aliquot plasma (leaving some above the cells) to a plastic centrifuge tube. 2. Centrifuge the aliquot tube. Pipette plasma (leaving some above the bottom of the tube) to another plastic aliquot tube. 3. Store plasma as required for the test ordered.
|
| Performed: |
1 day. Test set up Monday through Friday. |
| Reference value: |
Included on report. |
| Method: |
Automated Latex Immunoassay (LIA) |
| CPT Code: |
85246 |
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